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Biology 239
Spring 2012

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Please note that you are responsible for all information contained in this syllabus.

I.      Instructor:

Lee Couch
Office: Castetter 71 (Same floor as labs)
Phone: 277-2400
E-mail:lcouch@unm.edu

II.    Office Hours:

MWF 10:00-11:00, or by appointment.

III.   Course Description:

This is a course designed to serve as an introduction to the basic principles of bacterial physiology, virology, microbial parasitology, infection, pathogenicity, epidemiology, and host immunity. Review of basic biological principles will be minimal; therefore, it will be your responsibility to be sure that you are well-prepared for the course.

IV.   Course Objectives:

Students will:

• first and foremost, be able to think critically and apply the information given in class to everyday life problems.
• be able to differentiate types of bacteria based on cell structure.
• understand the various mechanisms of metabolism in prokaryotes.
• have a working knowledge of how bacteria grow and differ in growth requirements.
• be able to explain different methods (mechanical and chemical) to control growth of microbial organisms.
• be able to distinguish between genetic mechanisms and mutations of prokaryotes.
• learn the basic principles of immunology and virology.

V.    Text:

Microbiology: A Systems Approach, 3rd ed. M. Cowan. 2012. McGraw Hill Publ.

VI.   Prerequisites:

Biology 123 or 201 and Chemistry 111L or 121L (or equivalent).

VII.  Lab Requirement:

Biology 239 laboratory must be taken in conjunction with the lecture.

VIII.Grading:

The lecture grade will be based in part on 4 exams worth 50 pts. each. You may drop one of these four tests, but you must take the 4th exam to have it considered as one you want to drop. There is no outside extra credit given, but each test may have a bonus question at the end that can provide you with 2-4 extra credit points. Exams may include multiple-choice, matching, true/false, fill-in-the-blank, and short answer/essay style questions. Please allow at least one week for exams to be graded and returned.

There also will be 6 quizzes of 10 points each given randomly during the semester. You may drop one quiz, making them worth a total of 50 pts. Finally, the lecture portion of the class constitutes 75% of your total grade. The laboratory will make up the other 25%.

SINCE YOU CAN DROP ONE OF EACH, THERE WILL
BE NO MAKEUP EXAMS OR QUIZZES FOR ANY REASONS.

Breakdown:

4 Exams @ 50 pts. ea. (1 dropped) 6 Quizzes @ 10 pts. ea. (1 dropped) Total pts. for Lecture

=  150 pts. =    50 pts. =  200 pts.

Please keep the exams and quizzes that are returned to you as a record of your grades. In order to determine your grade in the lecture at any time during the semester, simply add all the points you have earned and divide that number by the total number of points possible up to that point. Overall final grades are based on percentages. UNM uses the fractionated grading system (e.g. A+, A, A-), so final grades will have +, -, or nothing depending on your score; but basically, grades are as follows:

Grade A+ A A- B+ B B- C+ C C- D+ D D- F

Percentage   99 - 100 93 - 98 89 - 92 86 - 88 83 - 85 79 - 82 75 - 78 69 - 74 65 - 68 62 - 64 59 - 61 55 - 58 54 and lower

Although roll is not taken, student attendance and participation is still required at every class meeting, so that you will understand the material and not fall behind. If you are absent for any reason, you are responsible for all information and/or materials presented in class, as well as any information regarding tests, schedule changes, etc. It is always a good idea to have someone who can lend you notes or provide you with any information you may have missed.

Finally, if you are having trouble with learning and understanding the material, labs, test taking, etc., please don't wait until close to the end of the semester to see me. I have office hours for answering questions and going over material you may be having trouble understanding. If you can't make my regular office hours, call or e-mail me to set up a time that will work for both of us to meet.





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Lecture Schedule--Spring 2010

Week	Date	Lecture	Chapter
1	Jan 18 Jan 20	Class Introduction/Review of Syllabus; Microbiology Introduction Finish Introduction; Review of Biological Macromolecules	1 2, handout
2	Jan 23 Jan 25 Jan 27	Techniques for Identification of Microorganisms Begin Prokaryote Cell Structure, Anatomy, Function Briefly review Eukaryote structure on your own Prokaryotes: Cell Structure--Gram + and -	3 + Notes 4 (pp.80-100) 4 (pp.80-100
3	Jan 30 Feb 1 Feb 3	Finish Prokaryote Cell Structure Energy & Metabolism: Brief Review of Enzymes, Glycolysis, Respiration Metabolism Review cont'd ***Feb 3--Last Day to Drop the Course w/o a Grade***	4 (pp. 80-100) 8 (pp. 198-225 8 (pp. 198-225)
4	Feb 6 Feb 8 Feb 10	Energy and Metabolism of Microbes: Metabolic Diversity Finish Metabolism; Brief Review for Test #1 Online Test #1 Review Exam #1 (Friday)	8 (pp. 198-225) 8 (pp. 198-225) ---
5	Feb 13 Feb 15 Feb 17	Dynamics of Microbial Growth Finish Microbial Growth Control of Growth	7 7 11
6	Feb 20 Feb 22 Feb 24	Control of Growth cont'd Brief Review of DNA Replication, Transcription and Translation Regulation of Gene Expression in Prokaryotes (Operons)	11 9 9
7	Feb 27 Feb 29 Mar 2	Mutations and Mutagens Microbial Genetics--Transformation, Transduction, Conjugation Finish Microbial Genetics; Review for Exam #2 Online Test #2 Review	9 9 9
8	Mar 5 Mar 7 Mar 9	Exam #2 (Monday) Taxonomy/Prokaryotes NO CLASS--Spring Break	--- 4 (pp 101-105) + Notes
9	Mar 12 Mar 14 Mar 16	NO CLASS--SPRING BREAK	--- --- ---
10	Mar 19 Mar 21 Mar 23	Prokaryotes cont'd. Eukaryotes: Parasitic Microorganisms Eukaryotes cont'd.: Parasites and Fungi	Notes 5 (pp. 121-135) 5 (pp. 121-135)
11	Mar 26 Mar 28 Mar 30	Viruses: History, Structure, Taxonomy, Types Viruses: Multiplication Viruses cont'd; Viroids, Prions (p. 450 only)	6 6 6
12	Apr 2 Apr 4 Apr 6	Disease Principles Disease Principles and Pathogenicity Finish Disease Principles; Begin Epidemiology	13 13 13
13	Apr 9 Apr 11 Apr 13	Epidemiology cont'd Finish Epidemiology; Review for Test #3 Online Test #3 Review Exam #3 (Friday) ***Apr 13--Last Day to Drop Course w/o Dean's Approval***	13 13 ---
14	Apr 16 Apr 18 Apr 20	Non-Specific Defenses Against Microbes Non-Specific Defenses cont'd; Begin Specific Defense Specific Defenses Against Microbes	14 14,15 15
15	Apr 23 Apr 25 Apr 27	Humoral Immunity Cell-Mediated Immunity Finish Immunity; Begin Antimicrobial Chemotherapy	15 15 15, 12
16	Apr 30 May 2 May 4	Antimicrobial Chemotherapy Finish Chemotherapy; Review for Exam #4 Online Test #4 Review Exam #4 (Friday)	12 12 ---






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Laboratory Schedule--Spring 2010

Lab Unit	Dates	Lab Exercises
1	Jan 18-20	Lab Safety--Appendix A Rules, Regulations, Equipment Introduction to Microbiology Lab Microscopes and Measurement Video: Humans and Bacteria
2 3	Jan 25-27	Media and Aseptic Transfer Technique Microbes in the Environment Hand Washing Effectiveness Introduction to Staining Simple Stains (Positive and Negative) Bacterial Morphology Quiz #1
4	Feb 1-3	Differential Stains: Gram Stain Students must master this staining technique! Review of other differential staining techniques Quiz #2
5 6	Feb 8-10	Introduction to Microbial Growth Selective and Differential Media Streak Isolation Technique Aerobic and Anaerobic Growth Catalase and Oxidase Tests Quiz #3
7	Feb 15-17	Gram Negative--Enterobacteriaceae I MacConkey Agar; Carbohydrate Metabolism; MR-VP Citrate; TSI Slants Streak Isolation practice Quiz #4
8	Feb 22-24	Gram Negative--Enterobacteriaceae II SIM; Ornithine and Lysine Decarboxylation Phenyalanine Deamination; Urease Video: Raw Terror--E. coli O-157 Quiz #5
9	Feb, Mar 29, 1, 2	Gram Positive Organisms (Staphylococcus) Catalase Test MSA Coagulase and DNase Tests Novobiocin Sensitivity Normal Skin Flora Explanation of Dichotomous Key & Flow Chart (Due in lab Mar 21-23) Quiz #6
10 11	Mar 7-9	Gram Positive Organisms (Streptococcus) Demo of different hemolytic Strep. species (alpha, beta, gamma) Bile Esculin Test CAMP Test Kirby-Bauer Antibiotic Sensitivity Antiseptics and Antibiotics Quiz #7
--	Mar 14-16	SPRING BREAK--NO LABS
--	Mar 21-23	Observe and record results from previous lab Turn in Flow Chart (10 pts.) Explanation of Unknown Exercise and Lab Report: Due May 2-4 Review for Practical Exam Quiz #8
--	Mar 28-30	LAB PRACTICAL
--	Apr 4-6	Primary Isolation of Unknowns
--	Apr 11-13	Isolation of Unknowns cont'd.
--	Apr 18-20	Isolation of Unknowns cont'd.
--	Apr 25-27	Finish Unknowns Video: Viruses--Attendance Required
--	May 2-4	Turn in Unknown Lab Reports Video--Attendance Required Quiz #9