---

Biology 239
Fall 2008

---

Please note that you are responsible for all information contained in this syllabus.
I.      Instructor:

Lee Couch
Office: Castetter 71 (Same floor as labs)
Phone: 277-2400
E-mail:lcouch@unm.edu

II.    Office Hours:

MWF 11:00-12:00, or by appointment.

III.   Course Description:

This is a course designed to serve as an introduction to the basic principles of bacterial physiology, virology, microbial parasitology, infection, pathogenicity, epidemiology, and host immunity. Review of basic biological principles will be minimal; therefore, it will be your responsibility to be sure that you are well-prepared for the course.

IV.   Course Objectives:

Students will:

• first and foremost, be able to think critically and apply the information given in class to everyday life problems.
• be able to differentiate types of bacteria based on cell structure.
• understand the various mechanisms of metabolism in prokaryotes.
• have a working knowledge of how bacteria grow and differ in growth requirements.
• be able to explain different methods (mechanical and chemical) to control growth of microbial organisms.
• be able to distinguish between genetic mechanisms and mutations of prokaryotes.
• learn the basic principles of immunology and virology.

V.    Text:

Microbiology: An Introduction, 9th ed. Tortora, Funke & Case. 2007. Benjamin/Cummings Publ. Co.

VI.   Prerequisites:

Biology 123 or 201 and Chemistry 111L or 121L (or equivalent).

VII.  Lab Requirement:

Biology 239 laboratory must be taken in conjunction with the lecture.

VIII.Grading:

The lecture grade will be based in part on 4 exams worth 50 pts. each. You may drop one of these four tests, but you must take the 4th exam to have it considered as one you want to drop. There is no outside extra credit given, but each test may have a bonus question at the end that can provide you with 2-4 extra credit points. Exams may include multiple-choice, matching, true/false, fill-in-the-blank, and short answer/essay style questions. Please allow at least one week for exams to be graded and returned.

There also will be 6 quizzes of 10 points each given randomly during the semester. You may drop one quiz, making them worth a total of 50 pts. Finally, the lecture portion of the class constitutes 75% of your total grade. The laboratory will make up the other 25%.

SINCE YOU CAN DROP ONE OF EACH, THERE WILL
BE NO MAKEUP EXAMS OR QUIZZES FOR ANY REASONS.

Breakdown:

4 Exams @ 50 pts. ea. (1 dropped) 6 Quizzes @ 10 pts. ea. (1 dropped) Total pts. for Lecture

=  150 pts. =    50 pts. =  200 pts.

Please keep the exams and quizzes that are returned to you as a record of your grades. In order to determine your grade in the lecture at any time during the semester, simply add all the points you have earned and divide that number by the total number of points possible up to that point. Overall final grades are based on percentages. UNM uses the fractionated grading system (e.g. A+, A, A-), so final grades will have +, -, or nothing depending on your score; but basically, grades are as follows:

Grade A+ A A- B+ B B- C+ C C- D+ D D- F

Percentage   99 - 100 93 - 98 89 - 92 86 - 88 83 - 85 79 - 82 75 - 78 69 - 74 65 - 68 62 - 64 59 - 61 55 - 58 54 and lower

Although roll is not taken, student attendance and participation is still required at every class meeting, so that you will understand the material and not fall behind. If you are absent for any reason, you are responsible for all information and/or materials presented in class, as well as any information regarding tests, schedule changes, etc. It is always a good idea to have someone who can lend you notes or provide you with any information you may have missed.

Finally, if you are having trouble with learning and understanding the material, labs, test taking, etc., please don't wait until close to the end of the semester to see me. I have office hours for answering questions and going over material you may be having trouble understanding. If you can't make my regular office hours, call or e-mail me to set up a time that will work for both of us to meet.





---

Lecture Schedule

Week	Date	Lecture	Chapter
1	Aug 25 Aug 27 Aug 29	Class Introduction/Review of Syllabus; Microbiology Introduction Finish Introduction; Biological Macromolecules NO CLASS, but review Biological Molecules	1 1, 2 2, handout
2	Sep 1 Sep 3 Sep 5	NO CLASS--Labor Day Holiday Microscopes and Staining Techniques Begin Prokaryote Cell Structure, Anatomy, Function Briefly review Eukaryote structure (pp 98-108) on your own	--- 3 4 (p.77-98)
3	Sep 8 Sep 10 Sep 12	Prokaryote Cell Structure: Gram + and - Finish Prokaryote Cell Structure Energy & Metabolism: Brief Review of Enzymes, Glycolysis, Respiration	4 4 5
4	Sep 15 Sep 17 Sep 19	Basic Metabolism Review cont'd. Energy and Metabolism of Microbes: Metabolic Diversity Finish Metabolism; Brief Review for Exam #1 Online Test #1 Review	5 5 5 ---
5	Sep 22 Sep 24 Sep 26	Exam #1 (Monday) Dynamics of Microbial Growth NO CLASS--but read and review Chap. 6	--- 6 ---
6	Sep 29 Oct 1 Oct 3	Finish Microbial Growth Control of Microbial Growth Brief Review of DNA Replication, Transcription and Translation ***Oct 3--Last Day to Drop the Course w/o a Grade***	6 7 8 ---
7	Oct 6 Oct 8 Oct 10	Regulation of Gene Expression in Prokaryotes (Operons) Mutations and Mutagens Microbial Genetics--Transformation, Transduction, Conjugation	8 8 8
8	Oct 13 Oct 15 Oct 17	Finish Microbial Genetics; Review for Exam #2 Online Test #2 Review Exam #2 (Wednesday) NO CLASS--Fall Break	8 --- --- ---
9	Oct 20 Oct 22 Oct 24	Taxonomy and Classification: Prokaryotes Prokaryotes cont'd. Eukaryotes: Parasitic Microorganisms	10 + Notes 10 + Skim 11 Skim 12
10	Oct 27 Oct 29 Oct 31	Eukaryotes cont'd.: Parasites and Fungi Viruses: History, Structure, Taxonomy, Types Viruses: Multiplication	Notes 13 13
11	Nov 3 Nov 5 Nov 7	Viruses cont'd; Viroids, Prions (p. 450 only) Disease Principles Disease Principles and Pathogenicity	13, 15 14 14, 15
12	Nov 10 Nov 12 Nov 14	Finish Disease Principles; Begin Epidemiology Epidemiology cont'd Finish Epidemiology ***Nov 14--Last Day to Drop Course w/o Dean's Approval***	14 14 14 ---
13	Nov 17 Nov 19 Nov 21	"Catch-up" and Review for Test #3 Online Test #3 Review Exam #3 (Wednesday) Non-Specific Defenses Against Microbes	--- --- --- 16
14	Nov 24 Nov 26 Nov 28	Non-Specific Defenses cont'd; Begin Specific Defense Specific Defenses Against Microbes NO CLASS--Thanksgiving Holiday	16, 17 17 ---
15	Dec 1 Dec 3 Dec 5	Humoral Immunity Cell-Mediated Immunity Finish Immunity; Begin Antimicrobial Chemotherapy	17 17 20
16	Dec 8 Dec 10 Dec 12	Antimicrobial Chemotherapy cont'd. Finish Antimicrobial Chemotherapy; Review for Exam #4 Online Test #4 Review Exam #4 (Friday)	20 20 --- ---






---

Laboratory Schedule

Lab Unit	Dates	Lab Exercises
1	Aug 27-29	Lab Safety--Appendix A Rules, Regulations, Equipment Introduction to Microbiology Lab Microscopes and Measurement Video: Humans and Bacteria
2 3	Sep 3-5 30-1	Media and Aseptic Transfer Technique Microbes in the Environment Hand Washing Effectiveness Introduction to Staining Simple Stains (Positive and Negative) Bacterial Morphology Quiz #1
4	Sep 10-12	Differential Stains: Gram Stain Students must master this staining technique! Review of other differential staining techniques Quiz #2
5 6	Sep 17-19	Introduction to Microbial Growth Selective and Differential Media Streak Isolation Technique Aerobic and Anaerobic Growth Catalase and Oxidase Tests Quiz #3
7	Sep 24-26	Gram Negative--Enterobacteriaceae I MacConkey Agar; Carbohydrate Metabolism; MR-VP Citrate; TSI Slants Streak Isolation practice Quiz #4
8	Oct 1-3	Gram Negative--Enterobacteriaceae II SIM; Ornithine and Lysine Decarboxylation Phenyalanine Deamination; Urease Video: Raw Terror--E. coli O-157 Quiz #5
9	Oct 8-10	Gram Positive Organisms (Staphylococcus) Catalase Test MSA Coagulase and DNase Tests Novobiocin Sensitivity Normal Skin Flora Explanation of Dichotomous Key & Flow Chart (Due in lab Oct 29-31) Quiz #6
---	Oct 15-17	NO LABS-FALL BREAK
10 11	Oct 22-24	Gram Positive Organisms (Streptococcus) Demo of different hemolytic Strep. species (alpha, beta, gamma) Bile Esculin Test CAMP Test Kirby-Bauer Antibiotic Sensitivity Antiseptics and Antibiotics Quiz #7
--	Oct 29-31	Observe and record results from previous lab Turn in Flow Chart (10 pts.) Explanation of Unknown Exercise and Lab Report Primary Isolation of Unknowns Review for Practical Exam Quiz #8
--	Nov 5-7	LAB PRACTICAL
--	Nov 12-14	Isolation of Unknowns cont'd.
--	Nov 19-21	Isolation of Unknowns cont'd,
--	Nov 26-28	THANKSGIVING HOLIDAY--NO LABS
--	Dec 3-5	Finish Unknowns Video: Viruses--Attendance Required
--	Dec 10-12	Turn in Unknown Lab Reports Video: Parasites--Attendance Required Quiz #9