Experiment 1 Results

Sample

d13C Frozen

d13C Preserved

d15N Frozen

d15N Preserved

d34S Frozen

d34S Preserved

Days in Formalin

Rhi 1

nd

nd

nd

nd

-13.0

-13.9

nd

Rhi 2

-25.16

-27.63

8.15

8.49

-13.0

-14.0

100

Rhi 3

-24.41

-27.17

7.90

7.90

-13.1

nd

100

Rhi 4

-24.95

-26.49

8.05

8.18

nd

nd

130

Rhi 5

-25.00

-27.20

9.30

9.70

nd

nd

10

Rhi 6

-22.95

-24.78

7.70

7.97

nd

-12.0

190

Rhi 7

-26.32

-27.53

9.24

9.74

-12.7

-13.0

160

Rhi 8

-25.15

-27.80

7.80

8.14

-13.1

-13.3

190

Rhi 9

-25.88

-28.91

8.61

9.19

-13.7

-13.2

70

Rhi 10

-24.87

-26.79

8.53

9.18

-13.2

-13.3

70

Rhi 11

-23.49

-24.82

7.90

8.38

-13.4

-12.2

10

Rhi 12

-24.96

-26.64

7.50

7.89

-13.5

-12.8

160

Rhi 13

-24.00

-25.87

7.68

7.97

-13.9

-13.7

160

Rhi 14

-24.57

-26.33

8.27

8.59

-11.9

-13.5

10

Rhi 15

-23.44

-25.70

8.33

9.27

-14.1

-14.3

130

Rhi 16

-23.62

-25.97

7.89

8.12

-12.8

-13.2

40

Rhi 17

-24.53

-26.80

7.48

7.97

nd

nd

190

Rhi 18

-25.98

-27.86

8.60

9.08

nd

nd

40

Rhi 19

-23.96

-25.37

8.58

9.01

nd

nd

40

Rhi 20

-23.83

-25.04

7.16

7.73

nd

nd

70

 

 

 

 

Experiment 2 Results

Sample

d13C Frozen

d13C Preserved

d15N Frozen

d15N Preserved

d34S Frozen

d34S Preserved

Log 1

-27.47

-29.11

16.44

17.42

-12.0

-11.2

Log 2

-27.10

-28.98

16.19

17.32

-11.5

-10.9

Log 3

-27.00

-28.57

15.41

15.74

-11.4

-10.0

Roa 1

-20.82

-21.54

12.43

12.43

-1.9

-0.5

Roa 2

-22.00

-21.46

13.22

13.02

0

-0.1

Roa 3

-21.96

-21.87

12.44

12.34

0

0.3

Tip 1

-24.71

-25.32

11.67

11.90

-10.9

nd

Tip 2

-24.65

-25.61

12.21

13.42

-11.2

-10.2

Tip 3

-24.93

-25.36

11.53

12.24

-11.4

-10.3

 

 

 

 

 

 

 

 

 

 

 

Experiment 3 Results

Sample

d13C Frozen

d13C Preserved

d13C Lipid Extracted

Rhi 1

-21.36

-22.29

-21.24

Rhi 2

-19.37

-20.99

-19.53

Rhi 3

-18.58

-19.72

-18.71

Rhi 4

-18.76

-20.46

-19.17

Rhi 5

-18.08

-19.27

-17.62

Rhi 6

nd

-20.38

-18.62

Rhi 7

-19.57

-20.90

-19.56

Rhi 8

-17.88

-20.33

-18.03

Rhi 9

-18.16

-19.81

-18.37

Rhi 10

nd

-19.92

-18.43

 

 

 

 

 

 

Experiment 4 Results

Sample

d13C - Mallinckrodt

d13C - JT Baker

d13C - EM Science

d13C Frozen

Cas 1

-23.51

-23.50

-23.71

-23.27

Cas 2

-23.31

-23.29

-23.71

-22.96

Cas 3

-23.72

-23.84

-24.15

-23.52

Cas 4

-22.25

-22.72

-22.75

-22.32

Cas 5

-23.00

-23.13

-23.28

-22.98

Cas 6

-23.21

-23.21

-23.66

-22.98

Cas 7

-23.78

-23.86

-23.87

-23.50

Cas 8

-23.20

-23.34

-23.59

-22.83

 

Rhi = longnose dace (Rhinichthys cataractae), Log = logperch (Percina caprodes), Roa = Roanoke darter (Percina roanoka), Tip = Tippecanoe darter (Etheostoma tippecanoe), Cas = white sucker (Castostomus commersoni).