Here are some molecular biology texts for reference:
Beginner's guide to Molecular Biology
Read this for background information on yeast:
Fred Sherman -Introduction to Genetics and Molecular Biology of Yeast
FRED SHERMAN Yeast Genetics- make sure you know these terms and processes::
auxotrophy - prototrophy
homozygous - heterozygous
essential - nonessential ORFs
deletion mutants
life cycle
cell cycle (mitosis)
sporulation (meiosis)
12 KB genome, 16 chromosomes
Table 6.1. Genetic nomenclature, using ARG2 as an example
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Gene: symbol Definition- --------------------------------------------------------------------------------
ARG+ All wild-type alleles controlling arginine requirement
ARG2 A locus or dominant allele
arg2 A locus or recessive allele confering an arginine requirement
arg2- Any arg2 allele confering an arginine requirement
ARG2+ The wild-type allele
arg2-9 A specific allele or mutation
Arg+ A strain not requiring arginine
Arg- A strain requiring arginine
Arg2p The protein encoded by ARG2
Arg2 protein The protein encoded by ARG2
ARG2 mRNA The mRNA transcribed from ARG2
arg2-D1 A specific complete or partial deletion of ARG2
ARG2::LEU2 Insertion of the functional LEU2 gene at the ARG2 locus, and ARG2 remains functional and dominant
arg2::LEU2 Insertion of the functional LEU2 gene at the ARG2 locus, and arg2 is or became nonfunctional
arg2-10::LEU2 Insertion of the functional LEU2 gene at the ARG2 locus, and the specified arg2-10 allele which is nonfunctional
cyc1-arg2 A fusion between the CYC1 and ARG2 genes, where both are nonfunctional
PCYC1-ARG2 A fusion between the CYC1 promoter and ARG2, where the ARG2 gene is functional