Levels of cellular organization (Biology texts)Different information exists at each level
Knowledge of all levels is required to model cells
- Genomics - a paradigm shift
- (HGP papers)(HGP analysis)
- New questions can be addressed through genomics
- (444/544 webpage links)
Microarrays: webpages-Argus, Nature Chipping Forcast; microarray database (France), Why we use logs,
- First developed to study RNA levels
- (Stanford Microarray Database)
- Two basic types - more on their way
- (Genome Biology)
Based on hybridization of complementary sequences
Detection methods (Axon)
Analysis (MAEtools, etc.)
The entire process:
- Array construction (protocols)
- Experimental conditions
- RNA isolation (protocol)
- Labelling (label/hyb protocols)
- Prehybridization and hybridization
- Scanning
- Data analysis (pages)
A systems approach:
- identification and quantitation of errors
- increased speed of analysis
- normalization - cross lab, cross platform
- integration of disciplines
- Where would good technology and biology get us?
- What can/could Sandia do?
