Dr. Eric (Sam) Loker
Office: 167F Castetter Hall
Lab Phone: (505) 277-3134
|
Dr. Eric (Sam) Loker | |
|
Office: 167F Castetter Hall |
Office Phone: (505) 277-2496 | |
| Lab: 265 Castetter Hall |
Lab Phone: (505) 277-3134 | |
| email: esloker@unm.edu | FAX: (505) 277-0304 | |
Research Interests: Schistosomiasis and schistosome biology; trematode–snail interactions;
comparative immunobiology; host–parasite
relationships.
CURRICULUM VITAE
EDUCATION:
Cornell University, Ithaca NY, B.S. Biology, 1972
University of Michigan, Ann Arbor MI, M.S. Zoology, 1974
Iowa State University, Ames IA, Ph.D. Immunobiology, 1979
POSITIONS:
Graduate Teaching Assistant, Department of Zoology, University of Michigan, 1972–74
Graduate Teaching Assistant, Department of Zoology, Iowa State University, 1974–78
Lecturer, Department of Zoology, Iowa State University, 1978
Instructor, Department of Zoology, Oregon State University, 1979–82
Postdoctoral Research Associate, Department of Zoology, Oregon State University, 1982–83
Assistant Professor, Department of Biology. Virginia Commonwealth University, 1983–85
Assistant Professor, Department of Biology, The University of New Mexico, 1985–90
Associate Professor, Department of Biology, The University of New Mexico, 1990–96
Fogarty Senior International Fellow, University of Glasgow, January–July, 1992
Regents' Lecturer, The University of New Mexico, 1994–97
Member, NIH Tropical Medicine and Parasitology Study Section, 1990, 1992, 1993, 1995, 1997, 2002Member, NIH study section on Tropical Medicine Research Centers, 2000, 2001
Chairman, NIH Special Emphasis Panel, Schistosomiasis Reagent Resource Center Proposal, 2002
Member, Editorial Board, Journal of Helminthology, Invertebrate Biology, 2004–present
Associate Chair, Department of Biology, The University of New Mexico, 1991–92, 1995–97
Professor, Department of Biology, The University of New Mexico, 1996–presentRegents' Professor, Department of Biology, The University of New Mexico, 2003–present
Chairman, Department of Biology, The University of New Mexico, 2003–present
Director, NIH COBRE Center for Evolutionary and Theoretical Immunology, 2003–present
Temporary Advisor, World Heath Organization Scientific Working Group on Schistosomiasis, 2005
PUBLICATIONS (since 1995):
Bandoni, S.M., Mulvey, M., and Loker, E.S. 1995. Phylogenetic analysis of eleven species of Biomphalaria Preston, 1910 (Gastropoda: Planorbidae) based on comparison of allozymes. Biological Journal of the Linnean Society 54:1-27.
Loker, E.S., and Adema, C.M. 1995. Schistosomes, echinostomes and snails: comparative immunobiology. Parasitology Today 11:120-124.
Bandoni, S.M., Mulvey, M., and Loker, E.S. 1995. Intraspecific and interspecific patterns of allozyme variation among species of Biomphalaria Preston, 1910 (Gastropoda: Planorbidae). Biochemical Systematics and Ecology 23:593-616.
Hanelt, B., Van Schyndel, D., Adema, C.M., Lewis, L.A., and Loker, E.S. 1996. The phylogenetic position of Rhopalura ophiocomae (Orthonectida) based on 18S ribosomal sequence analysis. Molecular Biology and Evolution 13:1187-1191. Abstract
Adema, C.M. and Loker, E.S. 1997. Specificity and immunobiology of larval digenean-snail associations. Pp. 229-263 in Advances in Trematode Biology, edited by B. Fried and T. K. Graczyk, CRC Press, Boca Raton.
Adema, C.M., Hertel, L.A., Miller, R.D., and Loker, E.S. 1997. A family of fibrinogen-related proteins that precipitates parasite-derived molecules is produced by an invertebrate after infection. Proceedings of the National Academy of Sciences, USA 94:8691-8696. Abstract
Hanelt, B., Adema, C.M., Mansour, M.H., and loker, E.S. 1997. Detection of Schistosoma mansoni in Biomphalaria using nested PCR. Journal of Parasitology 83:387-394. Abstract
DeGaffe, G. and Loker, ES. 1998. Susceptibility of Biomphalaria glabrata to infection with Echinostoma paraensei: Correlation with the effect of parasite secretory-excretory products on host hemocyte spreading. Journal of Invertebrate Pathology 71(1):64-72. Abstract
Mkoji, G.M., Mungai, B.N., Koech, D.K., and Loker, E.S. 1998. Experimental control of the schistosome-transmitting snail Biomphalaria pfeifferi by the ampullariid snail Pila ovata. Annals of Tropical Medicine and Parasitology 92(1):65-72. Abstract
Sapp, K.K., Meyer, K.A., and Loker, E.S. 1998. Intramolluscan development of the digenean Echinostoma paraensei: Rapid production of a unique mother redia that adversely affects development of conspecific parasites. Invertebrate Biology 117(1):20-28.
Mkoji, G.M., Boyce, T.G., Mungai, B.N., Copeland, R.S., Hofkin, B.V. and Loker, E.S. 1999. Predation of aquatic stages of Anopheles gambiae by the Louisiana red swamp crawfish (Procambarus clarkii). Journal of the American Mosquito Control Association 15(1):69-71. Abstract
Mkoji, G.M., Hofkin, B.V., Kuris, A.M., Stewart-Oaten, A., Mungai, B.N., Kihara, J.H., Mungai, F., Yundu, J., Mbui, J., Rashid, J.R., Kariuki, C.H., Ouma, J.H., Koech, D.K. and Loker, E.S. 1999. Impact of the crayfish Procambarus clarkii on Schistosoma haematobium transmission in Kenya. American Journal of Tropical Medicine and Hygiene 61(5):751-759. Abstract
Loker, E.S., Coustau, C., Ataev, G.L. and Jourdane, J. 1999. In vitro culture of rediae of Echinostoma caproni. Parasite-Journal De La Societe Francaise de Parasitologie 6(2):169-174. Abstract
Adema, C.M., Hertel, L.A. and Loker, E.S. 1999. Evidence from two planorbid snails of a complex and dedicated response to digenean (echinostome) infection. Parasitology 119(4):395-404. Abstract
Bandoni, S.M., Mulvey, M., and Loker, E.S. 2000. Population structure and taxonomic discrimination among three species of Biomphalaria Preston, 1910 (Gastropoda: Planorbidae) from Kenya . Zoological Journal of the Linnean Society 129:387-401.
Adema, C.M., Sapp, K.K., Hertel, L.A., and Loker, E.S. 2000. Immunobiology of the relationship of echinostomes with snail intermediate hosts. Pp. 149-173 in Echinostomes as Experimental Models for Biological Research, edited by Thaddeus K. Graczyk and Bernard Fried. Kluvyer Press.
Adema, C.M., Leonard, P.M., DeJong, R.J., Day, H.L., Edwards, D.J., Burgett, G., Hertel, L.A. and Loker E.S. 2000. Analysis of messages expressed by Echinostoma paraensei miracidia and sporocysts, obtained by random EST sequencing. Journal of Parasitology 86(1):60-65. Abstract
Hertel, L.A., Stricker, S.A., and Loker, E.S. 2000. Calcium dynamics of hemocytes of the gastropod Biomphalaria glabrata: Effects of digenetic trematodes and selected bioactive compounds. Invertebrate Biology 119(1):27-37.
Snyder, S.D. and Loker, E.S. 2000. Evolutionary relationships among the Schistosomatidae (Platyhelminthes: Digenea) and an Asian origin for Schistosoma. Journal of Parasitology 86:283-288. Abstract
Barber, K.E., Mkoji, G.M., and Loker, E.S. 2000. PCR-RFLP analysis of the ITS2 region to identify Schistosoma heamtobium and S. bovis from Kenya. American Journal of Tropical Medicine and Hygiene 62:434-440.
Sapp, K.K. and Loker, E.S. 2000. A comparative study of mechanisms underlying digenean-snail specificity: in vitro interactions between hemocytes and digenean larvae. Journal of Parasitology 86:1020-1029. Abstract
Sapp, K.K. and Loker, E.S. 2000. Mechanisms underlying digenean-snail specificity: The role of host hemocytes. Journal of Parasitology 86:1012-1019. Abstract
Snyder, S.D., Loker, E.S., Johnston, D., and Rollinson, D. 2001. The Schistosomatidae: Advances in phylogenetics and genomics. Pp. 194-200 in Interrelationships of the Platyhelminthes, edited by D.T.J. Littlewood and R.A. Bray. Taylor and Francis, London.
Zhao, X., Duszynski, D.W., and Loker, E.S. 2001. A simple method of DNA extraction for Eimeria species. Journal of Microbiological Methods 44:131-137. Abstract
Léonard, P.M., Adema, C.M., Zhang, S.M., and Loker, E.S. 2001. Structure of two FREP genes that combine IgSF and fibrinogen domains, with comments on diversity of the FREP gene family in the snail Biomphalaria glabrata. Gene 269:155-165. Abstract
Maldonado, A., Loker, E.S., Morgan, J.A.T., Rey, L., and Lanfredi, R.M. 2001. Description of the adult worms of a new Brazilian isolate of Echinostoma paraensei, (Platyhelminthes: Digenea) from its natural vertebrate host Nectomys squamipes by light and scanning electron microscopy and molecular analysis. Parasitology Research 87(10):840-848. Abstract
Zhao, X., Duszynski, D.W., and Loker, E.S. 2001. Phylogenetic position of Eimeria antrozoi, a bat coccidium (Apicomplexa, Eimeriidae), and its relationship to morphologically similar Eimeria spp. from bats and rodents based on nuclear 18S and plastid 23S rRNA gene sequences. Journal of Parasitology 87(5):1120-1123. Abstract
Ataev, G.L., Dobrovolskij, A.A., Avanessian, A.V., and Loker, E.S. 2001. Germinal elements and their development in Echinostoma caproni and Echinostoma paraensei (Trematoda) miracidia. Journal of Parasitology 87(5):1160-1164. Abstract
DeJong, R.J., Morgan, J.A.T., Paraense, W.L., Pointier, J-P., Amarista, M., Ayeh-Kumi. P.F.K., Babiker, A., Barbosa, C.S., Bremond, P., Canese, A.P., de Souza, C.P., Dominguez, C., File, S., Gutierrez, A., Incani, R.N., Kawano, T., Kazibwe, F., Kpikpi, J., Lwambo, N.J.S., Mimpfoundi, R., Poda, J-N., Sene, M., Velasquez, L.E., Yong, M., Adema, C.M., Hofkin, B.V., Mkoji, G.M., and Loker, E.S. 2001. Evolutionary relationships and biogeography of Biomphalaria (Gastropoda: Planorbidae) with implications regarding its role as host of the human bloodfluke, Schistosoma mansoni. Molecular Biology and Evolution 18(12):2225-2239. Abstract
Morgan, J.A.T., DeJong, R.J., Snyder, S.D., Mkoji, G.M., and Loker, E.S. 2001. Schistosoma mansoni and Biomphalaria: Past history and future trends. In Flukes and Snails, edited by D. Rollinson, a special supplement to the journal Parasitology 123:S211-S228. Abstract
Zhang, S-M., Léonard, P.M., Adema, C.M., and Loker, E.S. 2001. Parasite responsive IgSF members in the snail Biomphalaria glabrata: characterization of genes with tandemly arranged IgSF domains and a fibrinogen domain. Immunogenetics 53(8):684-694. Abstract
Loker, E.S. and Bayne, C.J. 2001. Molecular studies of the molluscan repsonse to digenean infection. Pp. 209-222 in Phylogenetic Perspectives on the Vertebrate Immune System, edited by G. Beck and E.L. Cooper. Kluwer Academic Publishers, Dortdrecht, The Netherlands. Abstract
Morgan, J.A.T., DeJong, R.J., Jung, Y., Khallaayoune, K., Kock, S., Mkoji,G.M., and Loker, E.S. 2002. A phylogeny of planorbid snails, with implications for the evolution of Schistosoma parasites. Molecular Phylogenetics and Evolution 25:477-488. Abstract
Hertel, L.A., Bayne, C.J., and Loker, E.S. 2002. The symbiont Capsaspora owczarzaki, nov. gen. nov. sp., isolated from three strains of the pulmonate snail Biomphalaria glabrata is related to members of the Mesomycetozoa. International Journal for Parasitology 32:1183-1191. Abstract
Pointier, J.P., Paraense, W.L., DeJong, R.D., Loker, E.S., Bargues, M.D., and Mas-Coma, S. 2002. A potential snail host of schistosomiasis in Bolivia: Biomphalaria amazonica Paraense, 1966. Memorias do Instituto Oswald Cruz 97:793-796. Abstract
Loker, E.S. and Nowak, T.S. 2003. Of genomes and parasitism. In Taxonomie, Ecologie et Evolution des Métazoaires Parasites. Taxonomy, ecology and evolution of metazoan parasites. (Livre hommage à Louis Euzet), Tome 2, edited by C. Combes and J. Jourdane. PUP Perpignan: 59-32.
Zhang, S-M. and Loker, E.S. 2003. The FREP gene family in the snail Biomphalaria glabrata: Additional members, and evidence consistent with alternative splicing and FREP retrosequences. Developmental and Comparative Immunology 27:175-187. Abstract
Morgan, J.A.T., DeJong, R.J., Lwambo, N.J.S., Mungai, B.N., Mkoji,G.M., and Loker, E.S. 2003. First report of a natural hybrid between Schistosoma mansoni and S. rodhaini. Journal of Parasitology 89:416-418. Abstract
Morgan, J.A.T., DeJong, R.J., Kazibwe, F., Mkoji, G.M., and Loker, E.S. 2003. A newly identified lineage of Schistosoma. International Journal for Parasitology 33:977-985. Abstract
DeJong, R.J., Morgan, J.A.T., Wilson, W.D., Al-Jaser, M.H., Appleton C.C., Coulibaly G., Doenhoff, M.D., Haas, W., Idris, M.A., Magalhães L.A., Moné H., Mouahid G., Mubila L., Pointier, J.-P., Webster J.P., Zanotti-Magalhães, E.M., Paraense W.L., Mkoji G.M., and Loker, E.S. 2003. Phylogeography of Biomphalaria glabrata and B. pfeifferi, the two most important hosts of Schistosoma mansoni in the New and Old World tropics. Molecular Ecology 12:3041-3056. Abstract
Mungai, B.N., Agola, L.E., Morgan, J.A.T., DeJong, R.J., Karanja, D.M.S., Muchemi, G.M., Loker, E.S., and Mkoji, G.M. 2003. Schistosoma rodhaini in Kenya revisited. Pp. 123-132 in Proceedings of Workshop on African Freshwater Malacology, September 2-12, 2003, Kampala, Uganda.
Loker, E.S., Adema, C.M., Zhang, S.-M., and Kepler, T.B. 2004. Invertebrate immune systems—Not homogeneous, not simple, not well understood. Immunological Reviews 198:10-24. Abstract
Nowak, T.S., Woodards, A.C., Jung, Y., Adema, C.M., and Loker, E. S. 2004. Identification of transcripts generated during the response of resistant Biomphalaria glabrata to Schistosoma mansoni infection using suppression subtractive hybridization. Journal of Parasitology 90:1034-1041. Abstract
Hertel, L.A., Barbosa, C.S., Santos, R.A.A.L., and Loker, E.S. 2004. Molecular identification of symbionts from the pulmonate snail Biomphalaria glabrata in Brazil. Journal of Parasitology 90:759-763. Abstract
Zhang, S.-M., Adema, C.M., Kepler, T.B., and Loker, E.S. 2004. Diversification of Ig superfamily genes in an invertebrate. Science 305:251-254. Abstract
Zhang, S.-M. and Loker, E.S. 2004. Representation of parasite-responsive genes encoding fibrinogen-related proteins (FREPs) in the freshwater mollusc Biomphalaria glabrata, an intermediate host for Schistosoma mansoni. Gene 341:255-266. Abstract
Hertel, L. A., Adema, C.M., and Loker, E. S. 2005. Differential expression of FREP genes in two strains of Biomphalaria glabrata following exposure to the digenetic trematodes Schistosoma mansoni and Echinostoma paraensei. Developmental and Comparative Immunology 29:295-303. Abstract
Pointier, J.-P., DeJong, R.J., Tchuem Tchuente, L.A., Kristensen, T.K., and Loker, E.S. 2005. A neotropical snail host of Schistosoma mansoni introduced into Africa and consequences for the schistosomiasis transmission: Biomphalaria tenagopphila in Kinshasa (Democratic Republic of Congo). Acta Tropica 93:191-199. Abstract
Nowak, T.S. and Loker, E.S. 2005. Echinostoma paraensei: Differential gene transcription in the sporocyst stage. Experimental Parasitology 109:94-105. Abstract
Lotfy, W.M., DeJong, R.J., Black, B.S., and Loker, E.S. 2005. Specific identification of Egyptian Biomphalaria species and possible hybrids using the polymerase chain reaction based on nuclear and mitochondrial loci. Molecular and Cellular Probes 19:21-25. Abstract
Loker, E.S. and Mkoji, G.M. 2005. Schistosomes and their snail hosts: The present and future of reconstructing their past. Chapter 1 in Schistosomiasis, edited by W. Evan Secor and Daniel G. Colley, World Class Parasites Series, Vol. 10, Springer.
Lotfy, W.M., DeJong, R.D., Kader, A.A., and Loker, E.S. 2005. A molecular survey of Biomphalaria in Egypt: Is B. glabrata present? The American Journal of Hygiene and Tropical Medicine 73:131-139. Abstract
Wilson, W.D., Johnson, P.T.J., Sutherland, D.R., Mone, H., and Loker, E.S. 2005. A molecular phylogenetic study of the genus Ribeiroia (Digenea): trematodes known to cause limb malformations in amphibians. Journal of Parasitology 91:1040-1045.
Jung, Y., Nowak, T.S., Zhang, S-M., Hertel, L.A., Loker, E.S., and Adema, C.M. 2005. Manganese superoxide dismutase from Biomphalaria glabrata. Journal of Invertebrate Pathology 90:59-63.
Brant, S.V. and Loker, E.S. 2005. Can specialized pathogens colonize distantly related hosts? Schistosome evolution as a case study. PLoS Pathogens 1:0167-0169. Article
Morgan, J.A.T., DeJong, R.J., Adeoye, G.O., Ansa, E., Barbosa, C.S., Brémond, P., Cesari, I.M., Charbonnel, N., Corrêa, L.R., Coulibaly, G., D’Andrea, de Souza, C.P., Doenhoff, M.J., File, S., Idris, M.A., Incani, R.N., Jarne, P., Karanja D.M.S., Kazibwe, F., Kpikpi, J., Lwambo, N.J.S., Mabaye, A., Magalhães, L.A., Makundi, Moné, Mouahid, G., Muchemi, G. M., Mungai, B. N., Séne, M., Southgate, V., Tchuem Tchuente, L.A., Théron, A., Yousif, F., Zanotti-Magalhães, E. M., Mkoji. G.M. and Loker, E.S. 2005. Origin and diversification of the human parasite Schistosoma mansoni. Molecular Ecology 14:3889-3902. Abstract
Brant, S. V., Morgan, J.A.T., Mkoji, G.M., Snyder, S.D., Rajapakse, R.P.V.J., and Loker, E.S. 2006. An approach to revealing blood fluke life cycles, taxonomy, and diversity: Provision of key reference data including DNA sequence from single life cycle stages. Journal of Parasitology 92:77-88.
Jiang, Y., Loker, E.S. and Zhang, S-M. 2006. In vivo and in vitro knockdown of FREP2 gene expression in the snail Biomphalaria glabrata using RNA interference (RNAi). Developmental and Comparative Immunology 30:855-866.
Adema, C.M. Luo, M.-Z., Hanelt, B., Hertel, L.A., Marshall, J.J., Zhang, S.-M., DeJong, R.J., Kim, H.-R., Kudrna, D., Wing, R.A., Soderlund, C., Knight, M., Lewis, F.A., Caldeira, R.L., Jannotti-Passos, L.K., dos Santos Carvalho, O., and Loker, E.S. 2006. A BAC library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni. Memorias do Instituto Oswaldo Cruz 101 (Supplement 1):167-177.
Loker, E.S. and Brant, S.V. 2006. Diversification, dioecy and dimorphism in schistosomes. Trends in Parasitology 22:521-528 (with cover).
Agola, L.E., Mburu, D.N., DeJong, R.J., Mungai, B.N., Muluvi, G.M., Njagi, E.N.M., Loker, E.S., and Mkoji, G.M. 2006. Microsatellite typing reveals strong genetic structure of Schistosoma mansoni from localities in Kenya. Infection, Genetics and Evolution 6:484-490.
Zhang, S.-M., Y. Zeng and E.S. Loker. 2007. Characterization of immune genes from the schistosome host snail Biomphalaria glabrata that encode peptidoglycan recognition proteins and gram-negative bacteria binding protein. Immunogenetics 59:883-898.
Stout, B.A., Adema, C.M., Zhang, S.-M., and Loker, E.S. In press, 2007. The biology of FREPs: Diversified lectins with fibrinogen-related domains from the freshwater snail Biomphalaria glabrata. Pages xxx-yyy ( Chapter 1) in Animal Lectins, Gerardo Vasta, ed.
Steinauer, M.L, L.E. Agola, L.E., Mwangi, I.N., Mkoji, G.M., and Loker, E.S. 2008. Molecular Epidemiology of Schistosoma mansoni: a robust, high-throughput method to assess multiple microsatellite markers from individual miracidia. Infection, Genetics and Evolution 8:68-73.
Lotfy, W.M., Brant, S.V., DeJong, R.J, Le, T.H., Demiaszkiewicz, A., Kinsella, J.M., Rajapakse, R.P.V.J., Perera, B.V.P., Laursen, J.R., Mkoji, G.M., and Loker, E.S. In press. Phylogenetic relationships among flukes of the Family Fasciolidae Railliet, 1895 with implications regarding their evolutionary biology and biogeography. American Journal of Tropical Medicine and Hygiene.
Steinauer, M.L., Mwangi, I.N., Maina, G.M., Kinuthia, J.M., Mutuku, M.W., Agola, E.L., Mungai, B., Mkoji, G.M., and Loker, E.S. In press. Interactions between natural populations of human and rodent schistosomes in the Lake Victoria Region of Kenya: A molecular epidemiological approach. PLoS Neglected Tropical Diseases.
Zhang, S.-M., Zeng, Y., and Loker, E.S. In review. Expression profiling and binding properties of fibrinogen-related proteins (FREPs), plasma lectins from the schistosome snail host Biomphalaria glabrata, revealed by antibodies raised from recombinant FREPs. Innate Immunity.
Steinauer, M.L., Hanelt, B., Ndungu, I., Maina, G., Lelo, E., Kinuthia, J., Wilson, W., Mungai, B., Mkoji, G., and Loker, E. Submitted. Introgressive hybridization of human and rodent schistosome parasites in western Kenya.
Agola, L.E., Steinauer, M.L., Mburu, D.N., Mungai, B.N., Mwangi, I.N., Magoma, G.M., Loker, E.S. and Mkoji, G.M. In preparation. Genetic diversity and population structure of Schistosoma mansoni within human infra-populations in Mwea, central Kenya assessed by microsatellites markers.
CURRENT FUNDING:
Title: COBRE: Center for Evolutionary and Theoretical Immunology; Principal Investigator: Eric S. Loker; Agency: NIH, National Center for Research Resources, P20 RR018754; Total Award: 5 years, direct costs, $7,732,,729; Duration: 30 September 2003–30 June 2008.Title: Evo-epidemiology of Schistosoma mansoni in Western Kenya; Principle Investigator: Eric S. Loker; Agency: National Institutes of Health; Total Award: 5 years, direct costs $1,497,748; Duration: 1 April 2004–31 March, 2009.
Title: Biology of Trematode–Snail Associations; Principal Investigator: Eric S. Loker; Agency: Competing Renewel, RO1 AI24340, National Institutes of Health; Total Award: 5 years, direct costs $1,250,000; Duration: 1 March 2006–28 Feb 2011.
Research
