Barbara Anne Stout, PhD

Office: 269 Castetter Hall, (505) 277-2743

Lab: 265 Castetter Hall, (505) 277-3134

Fax: (505) 277-0304

Mailing address: MSC03 2020

1 University of New Mexico

Albuquerque, NM 87131-0001

Education

B.S. (Medical Technology) 1984, Minnesota State University Moorhead

Ph.D. (Biomolecular Chemistry) 2003, University of Wisconsin-Madison

Employment

2006 – Present Scientist III, Biology Dept., University of New Mexico. Albuquerque, NM Research interests include host defense responses of the snail Biomphalaria glabrata, an intermediate host to a human pathogen Schistosoma mansoni, to infection by trematodes. Approaches taken include proteomics and microarrays.

2003 - 2006 Postdoctoral Fellow in the department of Pathophysiology, Lovelace Respiratory Research Institute, Albuquerque, NM

Research interests included airway inflammation and investigating the intracellular signaling pathways regulating biological processes such as apoptosis involved in maintaining homeostasis in the lung. Skills and approaches utilized include confocal fluorescent microscopy, molecular biology, immunohistochemistry, flow cytometry, mammalian cell culture, and protean analysis.

1997 - 2003 Research Assistant in the laboratory of Paul J. Bertics, Biomolecular Chemistry, University of Wisconsin-Madison, Madison, WI

The area of research focus was examining the effects of cytokines on eosinophil signaling and biology. Techniques and skills acquired and employed include microarray analysis, molecular biology, RNA work, flow cytometry, mammalian and insect cell culture, bacterial and Baculovirus system protein expression and purification, and protein analysis. The work performed was part of my PhD dissertation.

1988-1997 Senior Medical Technologist, Sonora Laboratory Sciences, Phoenix, AZ

1984-1988 Medical Technologist, Sonora Laboratory Sciences, Phoenix, AZ

Performance of clinical laboratory assays in a hospital/reference hybrid laboratory including in the departments of immunohematology, hematology, chemistry, and microbiology.

Professional Societies

American Thoracic Society

Graduate Women in Science

American Association for the Advancement of Science

American Society of Clinical Pathologists (ASCP)-associate member

Professional Certifications

Specialist in Blood Banking (SBB), American Association of Blood Banks, 1990

Medical Technologist (MT), American Society of Clinical Pathologists, 1984

Clinical Laboratory Scientist (CLS), National Credentialing Agency for Laboratory Personnel (NCA), 1984

Publications

Stout, B. A., Bhattacharya, S., Bates, M. E., Bertics, P. J. and Malter, J. S. (2001) GM-CSF and IL-5 activate STAT5 and induce CIS-1 mRNA in human peripheral blood eosinophils. American Journal of Respiratory Cell and Molecular Biology 24: 312-316.

Hall, D. J., Cui, J., Bates, M. E., Stout, B. A., Coffer, P., Koenderman, L. and Bertics, P. J. (2001) Transduction of human eosinophils with dominant negative H-Ras attenuates extracellular signal-regulated kinase activation and interleukin-5-mediated cell viability. Blood 98: 2014-2021

Bates, M. E., Liu, L. Y., Esnault, S., Stout, B. A., Fonkem, E., Kung, V., Sedgwick, J. B., Kelly, E. A., Bates, D. M., Malter, J. S., Busse, W. W. and Bertics, P. J. (2004) Expression of interleukin-5- and granulocyte macrophage-colony-stimulating factor-responsive genes in blood and airway eosinophils. American Journal of Respiratory Cell and Molecular Biology 30(5): 736-743.

Stout, B. A., Bates, M. E., Liu, L. Y., Farrington, N. N. and Bertics, P. J. (2004) Interleukin-5 and granulocyte macrophage colony-stimulating factor activate STAT3 and promote pim-1 and cyclin D3 protein expression in human eosinophils. Journal of Immunology, 173(10): 6409-17.

Stout, B. A., Rir-Sim-ah, J., Seagrave J., Xiang, J., Holtzman, M.J and Tesfaigzi, Y. IFNg causes a Stat1-associated Bax translocation to the endoplasmic reticulum to initiate cell death in airway epithelial cells. Submitted.

Stout, B. A., Adema, C. M., Zhang, S. M., Loker, E. S. The Biology of FREPs: Diversified Lectins with Fibrinogen-related Domains from the Freshwater Snail Biomphalaria glabrata. Submitted

Invited Oral Presentations

IFNg Causes a Stat1-associated Bax Translocation to Initiate Cell Death in Airway Epithelial Cells. Stout B. A., Rir-sim-ah J., Fischer M., Estrada I., Cardon K., Holtzman, M. J., Xiang, J. and Tesfaigzi, Y. American Thoracic Society 2005 Annual Meeting, San Diego, CA.

Abstracts

Stout, B. A. and Bertics, P. J. STAT3 and STAT5 are activated in human peripheral blood eosinophils following stimulation with IL-5 or GM-CSF. American Academy of Asthma, Allergy, and Immunology 2000 Annual Meeting, San Diego, CA. Journal of Allergy and Clinical Immunology 105(1): 272, Part 2, Suppl. S, 2000.

Stout, B. A. and Bertics, P. J. Effects of IL-5 and GM-CSF on STAT3 and STAT5 in human peripheral blood eosinophils. Graduate Women in Science 2000 National Conference, Madison, WI.

Stout, B. A. and Bertics, P. J. #21, STAT activation and CIS-1 induction in human eosinophils following cytokine stimulation. Signal Transduction Minisymposium 2000, Madison, WI.

Hall, D. J., Cui, J., Bates, M. E., Stout, B. A., Coffer, P., Koenderman, L. and Bertics, P. J. #3481, Transduction of a dominant-negative H-Ras into human eosinophils attenuates IL-5 mediated cell viability. American Society of Hematology 2000 Annual Meeting, San Francisco, CA.

Bates, M. E., Fonkem, E., Stout, B., Esnault, S., Busse, W. W. and Bertics, P. J. Interleukin-5 (IL-5) induces expression of lymphotoxin- (LT-) beta in human eosinophils. American Academy of Asthma, Allergy, and Immunology 2002 Annual Meeting, New York City, NY. Journal of Allergy and Clinical Immunology 109(1): 689 Suppl S, 2002.

Stout, B., Fonkem, E., Bates, M. E., Esnault, S., Busse, W. W. and Bertics, P. J. Interleukin-5 induces Pim-1 and cyclin D3 expression in human eosinophils. American Academy of Asthma, Allergy, and Immunology 2002 Annual Meeting, New York City, NY. Journal of Allergy and Clinical Immunology 109(1): 994 Suppl. S, 2002.

Stout, B. A., Pierce, J., Asmerom, T., Holtzman, M., and Tesfaigzi, Y. Activation of signal transducer and activator of transcription 1 (STAT1) by IFNg is sufficient to cause cell death in airway epithelial cells. American Thoracic Society 2004 Annual Meeting, Orlando, FL.

Stout, B. A., Rir-sim-ah, R., Seagrave, J.C., Tesfaigzi, Y. Interferon Gamma-Induced Apoptosis of Airway Epithelial Cells Involves STAT1 and Bax Translocation to the Endoplasmic Reticulum. American Thoracic Society 2004 Annual Meeting, San Diego, CA.