Isospora cruzi Pinto and Vallim, 1926

Type host: Scinax crospedospilus (Lutz, 1925) (syn. Ololygon crospedospila [Lutz, 1825] Fouquette and Delahoussaye, 1977), Campo Belo snouted treefrog.

Other hosts: Scinax fuscovarius (Lutz, 1925), Snouted treefrog; Scinax nasicus (Cope, 1862), Lesser snouted treefrog; Scinax ruber (Laurenti, 1768), Red snouted treefrog.

Type locality: SOUTH AMERICA: probably Brazil.

Geographic distribution: SOUTH AMERICA.

Description of oocyst: Oocyst shape: elongate-ellipsoidal, asymmetrical, being more pointes at one end than the other (line drawing from Carini, 1936); number of walls: 1; wall thickness: unknown; wall characteristics: thin and smooth (line drawing); L x W: 20.7 x 17.2 (20-22 x 17-18); L/W ratio: ~1.2; M: absent; OR: absent; PG: absent. Distinctive features of oocyst: asymmetrically ellipsoidal, more pointed at one end than the other.

Description of sporocysts and sporozoite: Sporocyst shape: subspheroidal; L x W: 14 x 13.8 (Pinto & Vallim, 1926) or 14 x 10 (Carini, 1936); L/W ratio: unknown; SB: absent; SSB: absent; PSB: absent; SR: present; SR characteristics: compact spheroidal mass of large and small granules. ~6-7, usually confined to one end of the sporocyst (line drawing); SZ: sausage-shaped without visible RBs or N (line drawing). Distinctive features of sporocysts: subspheroidal shape.

Prevalence: Unknown.

Sporulation: Presumably exogenous.

Prepatent and patent periods: Unknown.

Site of infection: Near the tips of the villi in the small intestine (line drawing).

Endogenous development: Mature meronts, spheroidal, 16–20, with 8–12 merozoites, each irregularly triangular, that assume the shape of a small rosette; each merozoite is 6–8 x 4–5. Microgametocytes are spheroidal, 15–18 wide, with each microgamete ~1.5 wide. Macrogamonts are spheroidal to ovoidal, 18–20 x 16– 18, with heavily granulated protoplasm.

Materials deposited: None.

Remarks: Pinto and Vallim (1926) found oocysts in the feces of several species of South American tree frogs, presumably from Brazil. Their oocysts were subspheroidal to ellipsoidal (line drawing), 20.7 x 17.2, with subspheroidal sporocysts, 13.8 x 14. Pinto and Vallim (1926) provided a line drawing of an unsporulated oocyst and a second drawing of an oocyst with 2 sporocysts filled with large globules, but there was no mention whether these were undeveloped sporocysts or if they were packed with a large SR. It was not until 1936 when Carini again found this species in S. ruber and documented that the two sporocysts actually had 4 SZ each. Carini (1936) also fixed some frog intestinal tissue for histological sections and described several of the endogenous stages.